LAG3 ELISA Kit

Produktnummer ABIN2043901

Human LAG3 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human LAG3.

Kurzübersicht für LAG3 ELISA Kit (ABIN2043901)

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails LAG3 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Marke: BioAssay™

Sensitivität: < 0.063 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Lymphocyte Activation Gene 3 (LAG3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Activation Gene 3 (LAG3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphocyte Activation Gene 3 (LAG3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphocyte Activation Gene 3 (LAG3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Lymphocyte Activation Gene 3 (LAG3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu LAG3

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Andere Bezeichnung: Lymphocyte Activation Gene 3 (LAG3)

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints